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This AMPK-Nrf2 axis is critical for maintaining redox balance and mitochondrial integrity in skeletal muscle ( In contrast, sustained or excessive ROS accumulation suppresses AMPK activity by destabilizing the AMPK-LKB1 complex, inducing inhibitory Ser485/491 phosphorylation via Akt/PKA signaling, and causing irreversible oxidative modifications of key cysteine residues (Figure 6